The p22phox gene -930A/G and A640G polymorphisms are not associated with essential hypertension in Iranian population: a case-control study

Back ground: Oxidative stress plays a crucial role in the pathophysiology of cardiovascular diseases such as essential hypertension. NADPH oxidase is the major source of reactive oxygen spices in the vascular system. Of various subunits of this enzyme, p22 phox is the key subunit to activation and stabilization of the NADPH oxidase. Among many variants of the CYBA, the gene which encodes p22phox, we chose to investigate the association between -930A/G in promoter and A640G in 3UTR of the gene with essential hypertension.Methods: In a case-control study in Iranian population, blood samples of 100 essential hypertensive patients and 100 normotensive controls were collected. The ARMS-PCR method was used for genotyping.Result: Data from this cross-sectional study showed the absence of an association between two polymorphisms and essential hypertension. In fact, there was not any difference in genotypic and allelic frequencies of the -930 A/G and A640G SNPs in case and control group.Conclusion: Our study showed that there is no association between -930 A/G and A640G polymorphisms of CYBA and essential hypertension in Iranian population. Although it seems that further studies with a larger population are needed to confirm these results.

Keywords:

Oxidative Stress Essential Hypertension , NADPH Oxidase ,