The association study of IGFBP3 -202 C> A polymorphism with breast cancer prevalence in Iranian females referred to Yazd Reproductive Sciences Institute

Background: Breast cancer included 33% of all cancers and 20% of cancer deaths in women. In Iran the fifth leading cause of death from cancer, is breast cancer. Several factors, including genetic factors involved in breast cancer.In recent years role of the Insulin-like Growth Factor gene family (IGF) in breast cancer is considered.Insulin-Like Growth Factor binding protein3 (IGFBP-3) is one of the most important members of IGF family. IGFBP-3 gene has various polymorphisms that affected increase and decrease of its serum level. One of its most common polymorphism, is rs2854744 that its association with breast cancer has studied in different populations, But so far has not been studied in Iranian women with breast cancer.Methods: 85 patients with breast cancer and 76 healthy female blood samples were collected and genomic DNA was extracted. The polymorphism was analyzed by PCR-RFLP method. Results showed no significant correlation between genotype and allele frequency of rs2854744 polymorphism with increased risk of breast cancer in the study population.Conclusion: Although this study showed no correlation between the rs2854744polymorphism and breast cancer risk but its association with disease in some other populations has been established. The difference may be due to the influence of other factors such as diet, physical activity, age and weight on circulating levels of IGFBP-3 and therefore risk of breast cancer.

Keywords:

breast cancer , rs2854744 , IGFBP-3 , PCR-RFLP