CIVILICA We Respect the Science
(ناشر تخصصی کنفرانسهای کشور / شماره مجوز انتشارات از وزارت فرهنگ و ارشاد اسلامی: ۸۹۷۱)

Association of single nucleotide polymorphism rs 3733961 APC gene related with has -miR– 155 in Gastric cancer

عنوان مقاله: Association of single nucleotide polymorphism rs 3733961 APC gene related with has -miR– 155 in Gastric cancer
شناسه (COI) مقاله: CIGS15_263
منتشر شده در سومین کنگره بین المللی و پانزدهمین کنگره ملی ژنتیک ایران در سال 1397
مشخصات نویسندگان مقاله:

Farinaz Soufastaei - Divition of cellular and Molecular biology ,r NourDanesh Institute of Higher Education,Meymeh , Iran
Banafshe Ashrafniya
Mansore Azade - Novin Biotechnology , Isfahan , Iran Department of Cellular Biotechnology Isfahan, Iran
Seyed Masoud Houshmand - National Institute of Genetic Engineering and Biotechnology ,Tehran, Iran
Kamran Ghaedi - Divition of cellular and Molecular biology , Department of Cellular Biotechnology Isfahan, Iran

خلاصه مقاله:
Gastric cancer is one of the most common cancer around the world[1]. Adenomatous polyposis coli or APC, has been known as a tumor suppressor gene. inactivation of APC causes the development of some gastric cancers, and mutations of the APC gene, occur during the early stages of gastric adenoma development [2] .The APC gene inhibits the members of Wnt signalling pathway that promotes β-catenin expression as a stimulator of cell division within the intestinal[2].MicroRNAs contribute to gastric carcinogenesis by altering the expression of oncogenes and tumor suppressors, affecting cell proliferation, apoptosis, motility, and invasion [3].material and method :miRNASNP2 database was used to identify the miRNAs with the ability to bind to the 3’UTR of APC transcripts. Then, miRTarbase and David were used to investigate the function and related pathways of obtained miRNA and relationship with APC. We, by kegg database, observed pathways in which miR-155 and APC are involved in cancers. Altogether, these stages helped us to find relationship between pathways of apc and mir-155.Results :In silico investigation of SNPs in the 3’UTR of APC gene showed that rs3733961 could alter the binding properties of miR-155. due to rs3733961, the binding activity of miR-155(as an tumor suppressor) undergoes loss; consequently, this SNP could act as a poor-prognostic factor. On the other hand, the binding affinity of miR-155 alleviates as a result of rs-3733961; therefore, rs-3733961 could also act as good-prognostic factor.Conclusion :Bioinformatically, rs3733961 could have association with gastric cancer especially with prognosis of patients.

صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/983834/