Non-invasive screening for miR-20a and miR92a can explain pathophysiological variations between COPD and Mustard Lung: Genetic or epigenetic involvement

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:

CIGS15_274

تاریخ نمایه سازی: 13 بهمن 1398

Abstract:

Background: Chronic obstructive pulmonary disease (COPD) and Mustard Lung (an illness resulting from Mustard gas used against Iranian victims by Iraqi forces) have many clinicopathological features in common making them difficult to distinguish. While COPD symptoms are gone after cessation of smoking, Mustard Lung symptoms progress by time throughout the decades after one-time exposure. While 20-30% of smokers develop COPD, only 1:50 of chemical patients progress severe stages of the disease demonstrating a stronger role of epigenetics in COPD compared to that of Mustard Lung.Objectives: Thus, to achieve a precise understanding of the molecular pathogenesis of these respiratory diseases and to bypass the problem of obtaining lung tissue samples, a non-invasive screening method of bio-molecules [micro-RNAs (miRNAs)] in bio-fluids (serum) was performed. We compared the relative expression of miR-20a and miR-92a (as representatives of the miR-17/92 cluster) in COPD and Mustard Lung patients.Methods: Stem-loop real-time quantitative polymerase chain reaction was employed for evaluation of miRNA expression. Student’s t-test and Mann-Whitney test were used for statistical analysis after performing one sample Kolmogorov-Smirnov (KS) test to check the normal distribution of samples (p<0.05). Results: A 8.6 and 97 folds of reduction was obtained, respectively for miR-20a and miR-92a in COPD patients relative to Mustard lung victims.Conclusions: Researchers confirmed the more intense role of epigenetics in COPD relative to Mustard lung observed at the bedside. This introduces innovative effective epigenetic strategies for treatment of respiratory diseases through regulating mir17/92 cluster as a target.

Keywords:

Sulfur Mustard , Chronic Obstructive Pulmonary Disease (COPD) , miR-20a , miR-92a

Authors

Houri Edalat

Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran