Comparison of Some Molecular Aspects of salt-induced Oxidative Stress in Aeluropus (Aeluropus littoralis L.) and Rice (Oriza sativa L.)
Publish place: The Third International and 15th National Genetics Congress
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
CIGS15_279
تاریخ نمایه سازی: 13 بهمن 1398
Abstract:
Salinity stress and high concentration of ions are of the most determinative factors that simultaneously affect on genetical, biochemical and physiological processes of a plant. Some of plants known as halophytes developed mechanisms that help them to avoid or tolerate the saline conditions. With the aim of understanding the probable mechanisms of saline tolerance or susceptibility in Aeluropus littoralis (as a halophyte) and Oriza sativa var. IR64 (as a glycophyte), we studied and compared some responses of their shoot and root to salt stress. To this end we established a stress span containing short term (6 hours), mid term (24 and 48 hours) and long term (5 and 11 days) exposure to salinity stress and their gene expression pattern and fluctuation of Cu/Zn Superoxide Dismutase (SOD), Catalase (CAT), cytoplasmic Ascorbat Peroxidase (cAPX), peroxisomal Ascorbat Peroxidase (pAPX), Glutathione Reductase (GR), and some of their nucleotide and amino acid sequence related indexes were investigated. Comparison of The real time quantitative PCR of ROS scavenger related transcriptom showed that Aeluropus increases the expression of these genes faster and keeps their expression in long-term while rice looses them significantly however with an exception in cAPX. Comparison of cDNA sequences and their counterpart amino acid codones also showed that Aeluropus has lower instability index and higher aromatic values with an exception for catalase amino acid sequence.
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Authors
Masoud Fakhrfeshani
Department of Plant Biotechnology, Jahrom University, Iran
Khalil Malekzadeh
Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Vail-e-Asr University of Rafsanjan, Rafsanjan, Iran
Mohammad Zare Mehrjerdi
Shirvan Higher Education Complex, Iran