Allelic variation of GSP gene in some wild wheat with A genome

The GSP protein belongs to a big family of seed storage protein in cereal seeds. Grain hardness has a great influence on the process properties of wheat to bread or biscuits. In this study we Re-sequenced the GSP gene in eight wild wheat accessions with A genome related to two spices (Triticum dicoccoides and T. urartu) that have been collected in the different region of Iran. The results of PCR showed that GSP gene is present in all of the examined samples and produced 570 bp, although the length of the PCR product on Agarose gel shows high polymorphism. The results of the GSP gene sequencing in samples indicated that Triticum diccocoides(73.8) and T. urarto (37.8) show highest and lowest Similarity with bead wheat at NCBI respectively. Indeed, the cluster analysis shows that the samples are divided into two main groups, although they could not separate the samples by species that have been collected from different region of Iran. In general, bioinformatics analysis showed that significant differences in the size and length of the Gsp gene in compare to NCBI sequence. In order to get a deep understanding of this variation in the wild genetic resources of Iran and western Iran, which is a potential source for the desired quality germs, it should be carefully studied via genetically and biochemical characteristic.

Keywords:

Wild wheat , Gsp gene , Quality