Decreasing of viability in Sodium Nitrite treated of ITPA down-regulated Human Umbilical Vein Endothelial Cells

ITPA gene has been identified as a DNA repair gene that maintains stability of the genome. DNA repair systems are essential for the maintenance of genome integrity. When repair proteins function is impaired through mutation, the genome may become unstable. Defects in ITPA can result in inosine triphosphatase deficiency, so inosine triphosphates (ITP) accumulate in the living cells. Sodium nitrite treatment induce Oxidative deamination that causes membrane damage, protein oxidation, lipid peroxidation and DNA damage. This study was focused on investigating the survival of ITPA down-regulated HUVEC compared to normal HUVEC in the presence of Sodium Nitrite. To evaluate the cell viability assay, we used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Briefly, 2x104 cells were incubated in a 96-well plate in the presence of various concentrations of sodium nitrite solution for 24–48 hours to determine the effect on endothelial cell proliferation. Sodium nitrite decreases proliferative activity in ITPA down-regulated compared to normal HUVEC cells. The proliferation of treated cells was significantly lower than in the control wells (p<0.05).

Keywords:

inosine triphosphatase(ITPA) , Sodium Nitrite , MTT , Cell Viability