Pathway analysis for immune response to CK-PAX3 virus using Path Visio

Recently, a novel protein in the influenza virus segment 3 has been identified, namely PA-X. This small protein has been reported to play a role in modulating host response of the H1N1 virus-infected mice. However, potential role of this protein in the pathogenicity and regulating host response of the highly pathogenic H5N1 virus in a chicken animal model is completely unknown. The aim of this study was to use of the microarray data for analyzing of biological pathways involved in response to influenza infection in chicken.The microarray data were obtained from ArrayExpress data bank (ebi.ac.uk/arrayexpress; Accession No: E-GEOD-53931). These data were related to a research to evaluate the global transcriptional response in the lungs of the chickens infected with the parental strain (CK10) and PA-X deficiency mutant strain (CK-PAX3). The quality of data was controlled using the facility available in ArrayAnalysis.org website. Path Visio 3.2.2 was utilized to perform pathway analysis. The number of genes on the pathways for CK-PAX3 that passed the criterion was 184. Six biological pathways had highest positive ɀ score and were ranked as most regulated. The results show that the biological pathways that are involved in cell apoptosis are highly active in influenza infection. The ACE Inhibitor Pathway, Apoptosis, Blood Clotting Cascade, Cholesterol Biosynthesis 1-7, EPO Receptor Signaling and FAS Pathway are included in this pathway. There are many genes that up/down regulated in these biological functions. It is necessary that the genes involved in apoptosis are investigated in details for their functions and pathways.

Keywords:

Influenza virus , PA-X , pathway analysis , microarray , chicken