Identification of two long non-coding RNAs differentially expressed in peripheral blood sample of diabetic patients with Real-time PCR

Diabetes mellitus (DM) is metabolic syndrome with high level of fasting blood sugar (FBS). Type 2 diabetes mellitus (T2DM) has been started in late age .The association between long non-coding RNAs (LncRNAs) and human diseases has been showed in many studies. Two LncRNA genes (ANRIL & PVT1) have been played an important roles in metabolic pathway and for this reason the aim of this study was to evaluate the expression of these two genes in peripheral blood sample of diabetic patients according to clinical and paraclinical data.Materials and methods: 75 peripheral blood samples of T2DM patients and 75 peripheral blood samples of non-diabetic people has been collected in CBC tubes with EDTA. Then total RNA was extracted and cDNA was synthesized. The expression of two genes (PVT1 & ANRIL) in T2DM patients compared to non-diabetic people was evaluated by Real-time PCR assay. Result: The expression of both genes examined in this study was significantly increased in T2DM patients compared to non-diabetic people.Discussion and conclusion:Due to significant overexpression of studied genes, they may be involved in process of T2DM. Also it is possible to use them as candidate genes for the diagnosis of diabetes pathogenesis.

Keywords:

ANRIL , PVT1 , LncRNA , Type 2 diabetes mellitus