The prediction of miR-429 and miR-557 role in azoospermia

Objective: Infertility in couples is a multifactorial complex problem, which has affected about 10-15% of the couples and half of cases due to male factors. Azoospermia or lack of sperm in each ejaculation is one of the infertility etiology in men. Azoospermia has many reasons. One of its causes is a disorder of spermatogenesis. Spermatogenesis is a phenomenon in which male sex cells are produced. It must be precisely regulated both in transcriptional and post-transcriptional stages. One of the major regulators of spermatogenesis are microRNAs. miRNAs are one class of noncoding RNAs that have about 21-25 nucleotides long and their function is gene silencing and regulation post-transcriptional and generally adjust the genes.Method: This is a theoretical study of bioinformatics. Significant genes that are essential for spermatogenesis were identified by using KEGG pathway database and corresponding literature mining and then by using databases such as miRwalk2.0, TargetScan, picTar and miRanda, interaction between the selected genes with miRNAs that their expression in azoospermia have been proven, were investigated. Results: According to the finding, miR-429 and miR-557 could inhibit spermatogenesis by inhibiting NANOS1 and DAZL genes respectively and probably causing disorders including azoospermia.Conclusion: Role of the miR-429 and miR-557 in inhibiting NANOS1 and DAZL genes and their prevention of spermatogenesis, maybe used as a therapeutic and pharmaceutical potentials as well as azoospermic identification biomarkers.

Keywords:

Azoospermia , Spermatogenesis , miR-429 , miR-557 , NANOS1 , DAZL.