Investigation of IL-17A gene expression and its polymorphism (rs3819025) in patients with Bullous pemphigoid

Bullous pemphigoid (BP) is an autoimmune skin disease, involving the formation of blisters, more appropriately known as bullae, at the space between the epidermis and dermis skin layers., some studies have shown an association between a IL-17A polymorphism (rs3819025) a mutation and BP disease susceptibility. Yet, these findings had so far not been independently replicated, and no data on a possible association of these mutations and BP in Iranian population were available.Methods This study contains 20 AA patients and 20 healthy controls. Genomic DNA was isolated using DNG-plus and PCR-RFLP analysis was performed to detect IL-17A rs3819025, polymorphism. Thereafter, cDNA was synthesized after RNA isolation. IL-17A expression levels were measured by Real-time PCR. Several relevant information such as demographic data (age, gender …) or clinical characteristics were analyzed for a possible effect of these factors on susceptibility to BP in patients.Results There was no significant difference in genotypes of IL-17A(rs3819025) polymorphism, in both patients and control groups(P> 0.05). IL-17A gene expression levels were significantly lower in patients with BP (0.709±0.49) in comparison with control individuals (2.14±0.36) (P=0.001). Conclusion This study did not showed an association between IL-17A(rs3819025) polymorphism and BP disease in Iranian population. Evaluation of IL-17A gene shows that the expression of this gene is significantly lower in patients than that of controls. This results shows that the genetic predisposition to develop BP can greatly varies among different ethnic groups.

Keywords:

Bullous pemphigoid , Autoimmune disease , IL-17-A rs3819025 , PCR-RFLP , Real-time PCR