Association of IL-6 polymorphism (rs1800795) with susceptibility to Bullous pemphigoid in Iran

Objective One of the most important of the autoimmune bullous skin diseases is Bullous pemphigoid (BP). Some studies have shown an association between IL-6 polymorphism (rs1800795) and BP disease susceptibility. These findings had so far not been independently replicated. In this study, a possible association of IL-6 polymorphism and BP in Iranian population was studied.Methods This study contains 20 BP patients and 20 healthy controls. Genomic DNA was isolated using DNG-plus and PCR-RFLP analysis was performed to detect IL-6 rs1800795 polymorphism. Furthermore, association of this IL-6 with some risk factors such as disease severity, family history, stress and the onset of the disease was assessed.Results No association between the IL-6 rs1800795 mutation and susceptibility to BP was observed in our Iranian population. PCR-RFLP results showed that no frequency of CC genotype are in both patient and control groups. CG genotype was detected in 40% and 45% of patients and controls, respectively. The GG genotype which is mutant genotype had higher frequency in patients and healthy individuals. Statistical analysis indicate that there no significant difference in distribution of genotypes between patients and controls (P> 0.05). The G allele had higher frequency of IL-6 in the patients and control subjects (80% vs. 77.5% respectively, P> 0.05). Conclusion We demonstrate that the IL-6 rs1800795 polymorphism and demographic factors in patients are not associated with the risk to develop BP in our Iranian population.

Keywords:

bullous skin diseases , demographic factors , IL-6 rs1800795.