Study of the suppressive effect of Nano-based compound of Gemini Curcumin on AGS Gastric cancer cell line

It has been shown that curcumin has effective anti-cancer properties. However, the absorption efficacy of curcumin is too low to have significant results in therapy. To overcome this problem, we first improved bioavailability of curcumin through making Nano-based compound of Gemini Curcumin, and then studied the effects of this compound on cell viability and apoptosis.Methods; Human gastric carcinoma AGS cell lines were treated with Gemini Curcumin(gCUR), and cell viability was evaluated using MTT assay. Cell cycle analysis was assessed by flow cytometry and also apoptotic potential of gCUR of were measured using Fluorescence microscope. Expression levels of some genes involved in apoptosis pathway such as bax and bcl2 were analyzed by qPCR.Results: Gemini curcumin inhibited the cell viability of AGS cells in a dose- and time-dependent manner (P ≤ 0.05) and arrested treated cell in G2/M phase. Our data also showed that the expression ratio of bax/bcl2 is increased in treated cell compared to controls.Conclusion: Gemini curcumin induces G2/M-phase arrest in human gastric cancer cells, which highlights its potent anti-cancer activity and potential application in gastric cancer therapy.

Keywords:

Curcumin , AGS cell line , Gastric cancer , bax/bcl2