Microarray Bioinformatic Analysis of Brain Tissue in Normal and Multiple sclerosis Patients

Background: Multiple sclerosis (MS) is an autoimmune inflammatory disease of the central nervous system. In this disease immune system attacks the myelin that covers nerve cell fibers and demyelination is happened. Both environment and genetic factors are involved in MS. However the cause of the disease is still unknown. The goal of the survey was to study differential gene expression between healthy control and active plaque.Material and methods: Microarray data of normal and patient brains was obtained from GEO dataset with GSE38010 and analyzed with R software (3.2.2 version) by affylmGUI package. Subsequently, up and down gene expression were proved by DAVID data base.Results: 5541 genes in tumor tissue were down-regulated and further 5279 genes were over expressed, compared to normal tissue. Thirteen genes with up regulation in this observation play a role in T cell receptor signaling pathway, acting as AP1 transcription factor which start transcription of inflammatory cytokines like TNF alpha.Conclusion: our finding could be important for MS pathogenesis and ethiology.

Keywords:

Multiple sclerosis , MS , GEO dataset whit GSE38010 , DAVID data base.