Reyhaneh Nassiri Mansour - Immunogenetics research center, Faculty of Medicine, Mazandaran university of Medical Sciences, sari, Iran
Abdolreza Ardeshirylajimi - Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Seyed Ehsan Enderami - Immunogenetics research center, Faculty of Medicine, Mazandaran university of Medical Sciences, sari, Iran

Background and Objective: Blood transfusion or blood products, such as plasma, have a long history in improving health, but today, platelet‐rich plasma (PRP) is used in various medical areas such as surgery, orthopedics, and rheumatology in many ways. Considering the high efficiency of tissue engineering in repairing bone defects, in this study, we investigated the combined effect of nanofibrous scaffolds in combination with PRP on the osteogenic differentiation potential of human dental pulp stem cells (DPSCs).Materials and Methods: Electrospinning was used for fabricating nanofibrous scaffolds by polyvinylidene fluoride/ collagen (PVDF/col) with and without PRP. After scaffold characterization, the osteoinductivity of the fabricated scaffolds was studied by culturing human DPSCs under osteogenic medium. The results showed that PRP has a considerable positive effect on the biocompatibility of the PVDF/col nanofibrous scaffold when examined by protein adsorption, cell attachment, and 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assays. In addition, the results obtained from alkaline phosphatase activity and calcium content assays demonstrated that nanofibers have higher osteoinductivity while grown on PRP‐incorporated PVDF/col nanofibers.Finding: These results were also confirmed while the osteogenic differentiation of the DPSCs was more investigated by evaluating the most important bone‐related genes expression level.Conclusion: According to the results, it can be concluded that PVDF/col/PRP has much more osteoinductivity while compared with the PVDF/col, and it can be introduced as a promising bone bio‐implant for use in bone tissue engineering applications

bone tissue engineering, collagen, dental pulp stem cells, platelet‐rich plasma, polyvinylidene fluoride