Validation and application of a biochip multi-array technology for the screening of 14 sulfonamide and trimethoprim residues simultaneously in milk

Milk is one of the main sources of supply of energy, protein and fat. Antimicrobial compounds are used in food production to treat or prevent animal diseases. The antibiotic residue in milk causes hypersensitivity reactions, and reduces quality of milk and other dairy products. Because of these concerns, many countries have banned or restricted the use of antimicrobial compounds in food-producing animals and have established maximum residue limits (MRL) for antimicrobial residues in foods. ThereThe Evidence Investigator™ Biochip Array Technology (Randox, UK) has been used as a screening method for simultaneous analysis of sulfonamides and trimethoprim residues in milk. In this study, the microarray kit I (AM I of Evidence Investigator™ system) for the simultaneous detection of 14 Sulfonamides (Sulphadiazine, Sulphadimethoxine, Sulphaquinoxaline, Sulphmethazine, Sulphmethoxazole, Sulphmethiazole, Sulphisoxazole, Sulpyridine, Sulphamerazine, Sulphamonomethoxine, Sulphamethoxypyridazine, The method was found to be rapid and able to screen simultaneously 14 sulphonamides and TMP in milk with no sample preparation procedure (or just one-step centrifugation). The false-positive rate of less 5% was obtained for all sulfonamides and TMP which is satisfactory. The result showed that for all antibiotics, the positivity threshold T was much higher than cut-off value Fm. Thus, all detection capabilities (CCβ) were equal to validated concentration and the CCβ were below the regulatory limTo our knowledge, the validation and application of biochip array technology for multiantibiotic residue screening has not yet been published. This application is, therefore, reliable and represents an innovative, fast, cost-effective, and multianalytical tool for Sulfonamides and Trimethoprim screening in about 45 samples at the same time.Due to high milk intake and the presence of different types of drug residues in this nutrient, it is essential to monitor other antibiotics in all types of dairy products in all provinces of Iran.

Keywords:

Validation biochip multi-array technology , sulfonamides and trimethoprim residues , milk , IR Iran