Molecular study of CTX-M-1, CTX-M2, CTX-M9, mcr-1, NDM-1 genes among Klebsiella pneumonia isolates from hospitalized patients in Taleghani hospital; Tehran, Iran
Publish place: 20th International Congress of Microbiology of Iran
Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
MEDISM20_071
تاریخ نمایه سازی: 26 بهمن 1398
Abstract:
Introduction and Objectives: Klebsiella pneumonia, is a major cause of hospital infections and the prevalence of drug resistance among them is increasing globaly. The aim of this study was molecular evaluation of CTX-M-1, CTX-M-2, CTX-M-9, mcr-1, NDM-1 genes among Klebsiella pneumonia isolates from hospitalized patients in Taleghani hospital. Materials and methods: Klebsiella pneumonia isolates from patients who referred to Taleghani hospital were collected during2016-2017.All were confirmed by standard bacteriologic tests. Antimicrobial susceptibility test (AST) was done by Kirby-Bauer method based on CLSI protocol. After DNA extraction, frequency of selected resistance genes was determined by PCR method and further sequencing. Statistical analysis was done by SPSS software. Results: among 70 isolates of Klebsiella pneumonia, the highest resistance were against: Ampicillin74%, Cefpodoxime65%, Ceftriaxone 57%, and 55.6% to Aztreonam. The most sensitivity was to Fosfomycin/Trometamol 86.5%, Amikacin, Imipenem, Doripenem, Meropenem, Ertapenem 71.6%. PCR results showed that 71% of the isolates had CTX-M-1 and 31% CTX-M-2, 23% CTX-M-9, 1.4% NDM-1. All were negative for mcr-1gene. Conclusion: with the release of new information on drug resistance and the detection of CTX-M 1,2, 9, mcr-1 and NDM-1 genes in Klebsiella pneumoniae isolates, doing molecular tests are recommended which may help physicians to prescribe the best and most appropriate antibiotic.
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Authors
Hadis Ghasemi
Dept. of Microbiology, Faculty of Basic Sciences, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
Mojdeh Hakemi-Vala
Dept. of Microbiology, Medical school, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Ali hashemi
Dept. of Microbiology, Medical school, Shahid Beheshti University of Medical Sciences, Tehran, Iran