Mandana Ohadi - Pharamceutics Research Center, Kerman University of Medical Sciences
Gholamreza Dehghannoudeh - Pharamceutics Research Center, Kerman University of Medical Sciences
Kioomars Kiani - Pharamceutics Research Center, Kerman University of Medical Sciences
Mohammad Hassan Moshafi - Pharamceutics Research Center, Kerman University of Medical Sciences
Negar Dehghannoudeh - Pharamceutics Research Center, Kerman University of Medical Sciences

Introduction and Objectives: optimizing the immobilization of pseudomonas aeruginosa as a biosurfactant producer was studied. Materials and Methods: Biosurfactant production by p. aeruginosa was confirmed through the hemolysis test, emulsification index (EI), and surface activity measurement. Calcium alginate encapsulation technique was used in order to entrap the P. aeruginosa cells. Full factorial design was employed to optimize bead preparation. Furthermore, the morphology and the stability of beads were evaluated. Results: It was proved that immobilized cells can be preserved the viability and biosurfactant production. Application of full factorial design indicated that the values of three parameters sodium alginate 3 %, CaCl2 1 % (w/v), and hardening time of 35 min, was found to be too stable for minimum surface tension and stable alginate gel beads. Conclusion: Finally, it could be concluded that the alginate gel beads showed good stability during the growing process and the immobilized cells efficiently were viable. Alginate source, hardening time and the interaction between CaCl2 concentration and hardening time influenced on the bead preparation.

Immobilization; Alginate Beads; Optimizing; Pseudomonas aeruginosa