In vitro evaluation of antifungal effects of Lactobacillus acidophilus and Lactobacillus plantarum compared to fluconazole against Candida glabrata isolated from oral cavity of HIV/ AIDS patients

Introduction and Objectives: Oropharyngeal Candidiasis (OPC) Known as an opportunistic Fungal Infections in immunosuppression human such as HIV/AIDS patients. C. albicans together other Candida species such as C. tropicalis, C. glabrata, C. krusei, C. kefyr, C. parapsilosis, and C. dubliniensis have been isolated from infected areas in the mouth. The aim of this study was in vitro evaluation of antifungal effects of L. acidophilus and L. plantarum compared to fluconazole (FLC) against C. glabrata isolated from oral cavity of HIV/ AIDS patients. Materials and Methods: Antifungal properties of both cells and CSFs of L. acidophilus and L. plantarum compared to fluconazole against C. glabrata investigated by Co-aggregation, agar overlay and interference and broth microdilution. Results: Our finding showed that both cells and CSFs of L. acidophilus and L. plantarum had antifungal effects against C. glabrata. The most intense inhibition was observed at low concentrations of L. acidophilus CFS compared to L. plantarum CFS and FLC (p <0.0001). In addition, significant difference was detected between antifungal effects of the CFS of two LABs (p <0.003). Conclusion: The antifungal effects of L. acidophilus and L. plantarum was higher than FLC on C. glabrata. Inhibitory effect of L. acidophilus CFS was more than L. plantarum CFS and FLC.

Keywords:

Oropharyngeal Candidiasis , Antifungal effects , probiotic , lactobacilli , L. acidophilus , L. plantarum , C. glabrata , AIDS/ HIV patients.