Isolation and molecular identification of Acetonitrile degrading bacteria from Jahrom city sewage

introductions and objectives: Environmental pollution is one of the most important problems facing the world today. These contaminations occur as a result of the absorption and accumulation of chemicals in the food chain and damage to the plants and living organisms of that climate. Nitrile is one of the most organic compounds in the Cyan active group, which is increasingly being produced in various natural and synthetic forms. Nitrile is uncontrollably entrained from sewage and sewage sludge and sewage and is considered as an important threat to the health of living organisms. The purpose of this study was to investigate and isolate the Acetonitrile degrading bacteria from Jahrom sewage. Materials and Methods: At first, the specimens were incubated at ambient temperature with Acetonitrile at 37 ° C for 14 days. After isolation and differential chemistry tests, MIC was used to isolate superior strains. Then strains were evaluated for growth kinematics and chromatographic analysis. At the end of the 16srRNA molecular tests and sequencing to identify the superior strains. Results: Among the 34 bacterial species of the two isolates, Pseudomonas otitidis and Bacillus subtilis were isolated in the shortest possible time, and the superior chromatographic analysis of these two bacteria showed that they were 92.35 and 7.35, Dissolves 80% of 0.1% concentration of Acetonitrile.

Keywords:

Acetonitril Biem idation , Pseudomonas otitidis , Bacillus subtilis , Parenteral bacteria