Rasoul Kaviani - Department of Medical Microbiology, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran.
Reza Mirnejad - Molecular Biology Research Center, System biology and Poisoning institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Iman Pouladi - Department of Medical Microbiology, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran.
Mohammad Niakan - Department of Medical Microbiology, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran.

Introduction: Acinetobacter baumannii is an opportunistic gram-negative bacterium that is commonly found in hospital infections, especially in the intensive care units (ICU). The present paper aims at Determination of the prevalence of Acinetobacter baumannii isolated from clinical samples by PCR molecular method in the city of Tehran.Materials and Method: In this study, 200 clinical samples from ulcer, pus, sputum, and blood were collected in Mostafa Khomeini, Tohid and Motahari hospitals in Tehran. The identity of the samples was verified by conventional standard biochemical tests and then by PCR and blaOXA-51-like gene amplification methods. Results: In this study, 60 isolates of Acinetobacter baumannii were identified based on biochemical differential tests, the identity of all of which were confirmed by blaOXA-51-like gene amplification through the PCR method. 25 samples were isolated from the ICU, 17 from infectious ward, 13 from the EMC, and 5 from other departments; by which, 25 blood samples (41.7%), 15 pus samples (25%), 12 ulcer samples (20%), and 8 urine specimens (13.3%) were obtained. Conclusion: Acinetobacter baumannii is one of the most important causes of hospital infections. The highest number of Acinetobacter baumannii-positive cases was related to ICU and infectious wards.

Acinetobacter baumannii, hospital infections, PCR