Evaluation of biofilm formation in Pseudomonas aeruginosa isolates

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:

MEDISM20_284

تاریخ نمایه سازی: 26 بهمن 1398

Abstract:

Introduction and objectives: Pseudomonas aeruginosa is an opportunistic pathogenic bacterium responsible for both acute and chronic infections. Beyond its natural resistance to many drugs, its ability to form biofilm, a complex biological system, renders ineffective the clearance by immune defense systems and antibiotherapy. The aims of this study was to determine the biofilm formation ability by P. aeruginosa clinical isolates. Materials and Methods: in this study, 99 clinical isolates of P. aeruginosa were studied. The ability of producing biofilm was examined by crystal violet microtiter plate assay and multiplex polymerase chain reaction (mPCR). Results: Among 99 isolated, 8 isolates formed strong biofilms, 12 isolates moderate, 47 isolates were formed weak biofilm and 31 isolates did not form biofilm. The PCR results on 8 isolates with strong biofilm showed that 8 isolates had genes cupA and lasR, 5 isolates had rhlR and 3 isolates had rhlI. Conclusion: The presence of genes encoding cell surface organelles, such as flagella and fimberial structure, and the genes that involved in quorum sensing system have a key role in biofilm formation. Infections with P. aeruginosa are a major problem for human health, and the understanding of the adhesive mechanisms used by the bacterium to colonize tissues as well as abiotic surface such as catheters or other medical devices is necessary to understand the infection process.

Authors

leila Dolatshah

Department of Pathobiology, School of Veterinary Medicine, Shiraz University

Mohammad Tabatabaei

Department of Pathobiology, School of Veterinary Medicine, Shiraz University