In vitro study on Nano zinc oxide’s bactericidal effect against the pathogenic agents of Iranian sturgeon infectious outbreaks

Introduction and Objectives: Previously, two bacteria, including Aeromonas veronii and Chrysobacterium joostei, were isolated from diseased beluga and stellate sturgeon which identified as the agents of severe mortality, and infectious symptoms. The present assay targets assessment of Nano zinc oxide (ZnO) s bactericidal effects against these bacterial pathogens in the form of In vitro study. Material & Methods: For this purpose, the physical features of Nano ZnO crystals were determined by X-ray diffraction (Shimadzu, XRD-6100). Afterward, the sensitivity of bacteria to the nanoparticle was measured via the well diffusion method. At the final stage, MIC was determined by the microdilution technique, whereas MBC was evaluated through cultivating of the samples of transparent wells on Muller Hinton agar. Results: XRD analysis demonstrated that Nano ZnO crystals’ diameter was estimated equal to 49.6 nm. According to the findings, both bacteria were sensitive to Nano ZnO, whereas they had intermediate resistance to florfenicol (reference antibiotic). The obtained MIC of ZnO for A. veronii and Ch. joostei were 0.06 and 0.03 μg/ml, respectively. Inhibitory effect of the assessed nanoparticle for our motile aeromonad was equivalent to the bacteriocidal trait (MBC). However, MBC of ZnO for the cytophaga was recorded twice as much as its MIC (about 0.06). Conclusion: The bactericidal/ inhibitory impact of Nano ZnO recognized well-suited for control of these pathogenic agents at In vitro level. It seems that necessary to use this nanoparticle in the sturgeon farms, after pass to In vivo and toxicity tests.

Keywords:

Nano zinc oxide (ZnO) , Sturgeon , Aeromonas veronii , Chrysobacterium joostei , XRD analysis.