In vitro Eradication of Pseudomonas aeroginosa Persister Cell Producers by Herbal Medicine Running title: Anti-persister of Peganum harmala

Introduction and Objectives : Unfortunately, today multidrug resistant (MDR) bacteria are very common in communities and could serious threat to human health. However, new mechanism of survival of bacteria without any resistance is debatable. One of the most important mechanisms is persister cell formation in bacteria. Persister cell can be one of the important causes in chronic infections. Nevertheless, there are many challenges in the treatment of persister cell. The discovery of new drugs and appropriate therapies in this area is very necessary. So, the purpose of this research was discovering an antipersister drug in Pseudomonas aeruginosa clinical isolates. Materials and Methods :Thirty P. aeruginosa clinical isolates were used. Antibiotic susceptibility testing was done and persister cell assay was performed. Finally, P. harmala was used as an anti-persister cell drug for in this isolate. Result :Our finding demonstrated that concentration of 35ug/ml completely has the anti-persister properties. Conclusion :So, it can be herbal candidate against persister cells but further analysis including in vivo study and finding the main component should be done to commercial this herb as new anti-persister drug.

Keywords:

persister cell , Peganum harmala , pseudomonas aeruginosa