Detection of Canine Herpesvirus in vaginal samples collected from dogs in Kerman city by Real Time-PCR

Introduction and Objectives: Canine Herpesvirus is belonged to the family of herpesviridae and has been detected in various countries worldwide. This virus can result in reproductive disorders, such as abortion stillbirth and week puppies. Virus transmission is mainly through the placenta, birth canal, sexual contact and respiratory tract. Considering the global prevalence of this virus and its economic importance due to abortion and stillbirth for dog breeders, this research was conducted to determine the prevalence of CHV1 in dogs of Kerman. Materials and Methods: In this study, the presence of herpes virus in vaginal swab specimens and biopsy of the uterus of dogs referring to Veterinary Hospital of Shahid Bahonar University of Kerman and some breeding kennels of Kerman city were determined. Samples were collected and evaluated using Real-Time PCR. Result: Canine Herpesvirus type 1 was detected in 21 samples from a total of 140 collected samples (7/70 vaginal swabs and 14/70 uterine biopsies) from referred dogs and 21 out of 64 vaginal samples of breeding dogs. Conclusion: Considering the significant prevalence of this virus, it is necessary to carry out management measures in control and prevention this disease. Developing of proper diagnostic method for rapid screening of infected dogs is highly recommended.

Keywords:

Canine Herpesvirus , Reproductive samples (Uterine and vagina) , Dog , Kerman