Study of sarcocyst contamination rate by using histopathology and molecular method in carcasses of cattle slaughtered in Isfahan

Introduction and Objectives: Sarcocystis is one of the most common parasites in domestic animals. The parasite is important in terms of economic and pathogenicity in domestic animals. Materials and methods: In this study, the esophageal muscles, diaphragm and the heart of 100 slaughtered cows at the isfahan slauphter-house were collected which contamination was investigated based on age, sex, season and infected organs. macroscopic cysts were found by studing of the carcasses and specimens in 7 cows. Microscopic cysts of Sarcocystis were detected by histopathologic method and by staining with hematoxylin-eosin and bradyzoites under a microscope. Results: The rate of infection was found as below: in the heart (44%); in the esophagus (30%); and in the aperture (12%). Conclusions: Heart was recognized as the most contaminated tissue. Also, the highest infection was between the ages of 2-7 years old and mostly appear in the summer. Femal are most faced with contaminations.DNA extracting and refining was performed by using kits and PCR conditions were provided for replication of 18 s R-rna fragment was provided. The PCR evaluation showed that microscopic cysts belong to the genus sarcocyst.

Keywords:

sarcocyst , slaughter-house , PCR , contamination organ