ASSESSMENT OF A NEW NANOSENSOR FOR PARACETAMOL SCREENING IN EMERGENCY DEPARTMENT

Background and Aim : Paracetamol is commonly taken in acute overdose. Here, we report arapid and sensitive nanosensor for detection of the paracetamol in blood.Methods : This Qualitative assay kit was designed to change its color at the three levels of Plasmaconcentration of acetaminophen as follows: dark red for Serum level above 100mcg/ml (high risk),red for Serum level between 25 -100mcg/ml (intermediate risk) and pink for Serum level below25mcg/ml (low risk) was contracted. Acute Acetaminophen over dose, induced in rats by the oraladministration of a single dose of 2 g/kg N-acetyl-para-aminophenol (APAP). The relationshipbetween liver enzymes and serum acetaminophen levels which determined by the kit wasinvestigated.Results : One hour after the administration of toxic dose, 73 rats, had serum levels above100mcg/ml (group A), 22 rats, had serum levels between 25-100 mcg/ml (group B) and 5 rats, hadserum levels less than 25 mcg/ml (group C). Aspartate transaminase (AST) was measured in groupA, B and C, 106.22±1.05, 100.15±0.00 and 108.71±1.01 (IU) respectively, 2 hours after toxic doseadministration. The same way, Alanine transaminase (ALT) was measured in group A, B and C,32.54±0.59, 32.76±0.59 and 32.68±0.54 (IU) respectively. There was no significant differencebetween the groups. 5 hours after the administration of toxic dose, AST was measured in group A,B and C, 274.49±1.27, 173.17±2.22 and 134.85±2.31 (IU) respectively and also ALT wasmeasured in group A, B and C, 156.5±0.00, 93.91±0.23 and 44.54±0.91 (IU) respectively. Therewas a significant difference in the level of liver enzymes in the group A, which was reported highrisk by nanosensor.Conclusion : This point-of-care test could be used to rule out an overdose with acetaminophen,and could thus lead to earlier clinical decisions for suspected overdose patients.

Keywords:

Nano sensor , paracetamol , screening test , liver enzymes , Rat