The levels of plasma glucose and insulin; oxidative stress and body weight in streptozotocin induced diabetic rats treated with aqueous solution of Moracin

The aim of present attempt was to determine possible therapeutic influence of provision of oral Moracin on levels of plasma glucose and insulin; oxidative stress and body weight in the streptozotocin-induced diabetic rats. The well known classical biochemical methods were used for the determination of plasma malondialdehyde (MDA), nitric oxide (NO), glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH) levels. Enzyme-linked immunosorbent assay (ELISA) was used for the determination of levels of insulin in plasma. The reverse transcriptasepolymerase chain reaction was used for the determination of the levels of brain antioxidant enzymes (SOD, CAT, and GSH-Px). The diabetes-related increase in blood glucose levels was found reduced through the provision of Moracin over the period of eight weeks. The levels of plasma nitric oxide (NO) and glutathione (GSH) of brain tissue were found significantly reduced in the Moracin treated group of streptozotocin-induced diabetic rats. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) in the hemolysate samples from Moracin treated streptozotocin-induced diabetic ratswere observed significantly increased in comparison with untreated to the group of streptozotocininduced diabetic rats. The superoxide dismutase (SOD) and catalase (CAT) activities in the homogenates of brain tissue of Moracin treated streptozotocin-induced diabetic rat did not exhibited the significant changes. whereas glutathione (GSH) activity was found increased in the Moracin treated group in comparison with untreated to the group of streptozotocin-induced diabetic rats. The superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) mRNA transcription levels were suppressed in the diabetic group compared to the control. The suppression in superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) mRNA transcription was found stopped through the provision of Moracin. The oxidative damage and low levels of insulin associated with diabetes were ameliorated with the Moracin treatment. The Moracin, the novel compound belong to the mulberry, Morus alba (L.) should be introduced in the human diet. The Moracin deserve effectiveness for the diabetic patients.

Keywords:

Diabetes induced xidative stress , Moracin , Mulberry , Morus alba (L.).