Anti-Cancer Activity of Methanol Extracts of Cichorium Intybus on Human Breast Cancer SKBR3 Cell Line

Background: Breast cancer is the most prevalent cancer and the second cause of death among women around the world. In manycancers, including breast cancer, Fatty acid synthase (FASN) gene expression is increased significantly. In breast cancer cell lines,expression of FASN is higher in HER2 positive cell line like SKBR3 than the others. FASN is the key enzyme for fatty acid synthesisde novo pathway and it is producing palmitate which is necessary for cell membrane formation. Cichorium intybus is a medicinalplant that effectively leads to inhibition of fatty acid synthase and thus reduces the percentage of survival of cancer cell lines.Objectives: The aim of this study was to evaluate the effect of methanol extract of Chicorium intybus root on percentage of survivalin SKBR3 cell line.Methods: Human breast cancer SKBR3 cell line was cultured in DMEM medium. Methanol extract of Cichorium intybus root wasextracted and different dilutions (200, 300, 400, 500 and 600 g/mL) were added to cell culture. Cell viability was quantitated byMTT assay after 24, 48 and 72 hours.Results: Cichorium intybus could decrease cell viability. The effects of extract on cell viability were observed after 24, 48 and 72hours on SKBR3 cell line and IC50 was 800, 400 and 300 after 24, 48 and 72 hours of treatment, respectively.Conclusions: Our study shows that methanol extract of Cichorium intybus has cytotoxic effects on tumor cells. This is a pilot workfor further evaluation in the future.

Keywords:

Cichorium Intybus , SKBR3 Cell Line , Fatty Acid Synthase , MTT Assay , Cell Viability