Chandrajeet Kumar - Department of Environment and Water Management, A.N. College, Patna, India
Ashok K.Gosh - Bihar State Pollution Control Board, Patna, India

Purpose Vemifiltration units are sludge-free, noise-free and low- or no-electricity-requiring systems of operation. The aimof this study was to emphasize wastewater treatment by vermifiltration technology using waste-eater earthworms to highlightthe benefits of clean and nutritive vermifiltered water (vermiaqua) uses in agriculture to the farmers.Methods Wastewater sample was filtered through fabricated vermifiltration unit to study physio-chemical and biologicalproperties of vermiaqua and uniform-sized Allium cepa (Onion) bulbs were kept for root germination at different concentrations(10−1, 10−3, 10−5, 10−7, 10−9) of wastewater and vermiaqua at room temperature for 5 days to study impacts ofvermiaqua on morphological and cytological characteristics of onion.Results Vermiaqua was odorless, pale yellow in appearance, lower in turbidity and highly nutritive as ammonia and nitratecontents were highly increased. In E. coli-free vermiaqua, BOD5loads were also reduced by 91%. Almost all vermiaquatreatedroot germinations were ‘accelerated’, whereas all wastewater-treated root germinations were ‘retarded’. The highestnumber of germinated roots was counted at 10−5 concentration of vermiaqua, whereas at the same concentration of wastewaterno germination was observed. Many types of chromosomal abnormalities were observed at metaphase and anaphasestages of wastewater-treated roots, whereas the single type of chromosomal abnormality was observed at anaphase stage ofvermiaqua-treated roots.Conclusions Earthworms upgraded the performance of fabricated systems having integrated methods (biological, chemicaland physical) of wastewater purification. Morphological and cytological studies revealed vermiaqua highly promoted theroot germination without any chromosomal abnormalities.

Allium cepa · Chromosomal abnormalities · Earthworms · Vermifiltration · Vermiaqua · Wastewater