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Title

Real Time RT PCR Detection of HCN4 and ADAM8 Genes in Ventilator Associated Pneumonia Patients who Hospitalized inIntensive Care Unit

Year: 1395
COI: JR_SBMU-1-4_003
Language: EnglishView: 200
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Authors

Hamidreza Jamaati - Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
Naghmeh Bahrami - Craniomaxillofacial Research center, Tehran University of Medical Sciences, Tehran, Iran. Oral and Maxillofacial Surgery Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Masoum Abniki - Department of Biotechnology, Faculty of Sciences, Islamic Azad University, Tehran, Iran
Payam Tabarsi - Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
Behrooz Farzanegan - Tracheal Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
Atosa Doroudinia - National Research Institute for Tuberculosis and Lung Disease, Department of Pathology Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract:

Background: Ventilator-associated pneumonia (VAP) is one of the most serious and prevalent nosocomial infections among patients in the intensive care unit (ICU), which is often recognized after detecting the symptoms. To date, there is no proper clinical and diagnostic marker for early detection of this disease. In this study, two genes (HCN4 and ADAM8) were assessed to be used as biomarkers for recognition of the disease in patients with VAP.Materials and Methods: This study was a case control study, which was done in Masih Daneshvari Hospital affiliated to Shahid Beheshti University of Medical Sciences, Tehran, Iran, since 2015-2016. Current study consisted of 30 patients with VAP and 30 Healthy people. After extraction of RNA, cDNA synthesis was done. Expression of genes was assessed by Real time PCR.Results: In peripheral blood samples of the patients, 10 out of 30 did not have positive HCN4 gene expression. Among the healthy individuals, 6 out of 30 cases did not have positive HCN4 gene expression. Moreover, in ADAM8marker patients, 13 out of 30 did not have positive ADAM8 gene expression and 8 out of 30 healthy individuals did not have positive ADAM8 gene expression.Conclusion: Genes HCN4 and ADAM8 assessment with Real Time-PCR in this study can be used as promising markers in early detection of VAP disease. More extensive studies on larger sample sizes may yield higher sensitivity for these molecular markers.

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This Paper COI Code is JR_SBMU-1-4_003. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/994796/

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Jamaati, Hamidreza and Bahrami, Naghmeh and Abniki, Masoum and Tabarsi, Payam and Farzanegan, Behrooz and Doroudinia, Atosa,1395,Real Time RT PCR Detection of HCN4 and ADAM8 Genes in Ventilator Associated Pneumonia Patients who Hospitalized inIntensive Care Unit,https://civilica.com/doc/994796

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Type of center: علوم پزشکی
Paper count: 5,531
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