Micropropagation of Persian walnut (Juglans regia L.) by embryo culture

One of the new and important ways of plant propagation is micropropagation or tissue culture technique. Using this technique is preferred more due to its vast propagation of plants in short periods. This research presents an investigation on media and culture conditions for in vitro proliferation of Persian walnut (Juglans regia L.). Prepared embryo segments (explants) produced callous and new shoot and root on WPM (woody plant medium) with and without the usage of IBA (Indole Butyric Acid) hormone. An experiment was carried out under conditions of 25ºC, light/dark period of 16 and 8 hours and a light intensity of 3000 lux. Results showed that root and shoot proliferation percentage on WPM supplemented with 0.5mg IBA hormone were 70 and 60 percent respectively, whereas without using IBA the these data were less than 50 percent. How ever, after the end of experiment about 60% of replications resulted to seedling (completed plant). Comparing medium treatments indicates that the appliance of IBA improved and promoted micropropagation of walnut.

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Payame Noor University , Gonabad Centre