CIVILICA We Respect the Science
Publisher of Iranian Journals and Conference Proceedings
Paper
title

Genetic diversity of a vaccine candidate gene encoding Plasmodium vivax Duffy Binding Protein-II for employing in vaccine development investigations

Credit to Download: 0 | Page Numbers 1 | Abstract Views: 704
Year: 2014
COI code: CIGS13_0872
Paper Language: English

How to Download This Paper

For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.

Authors Genetic diversity of a vaccine candidate gene encoding Plasmodium vivax Duffy Binding Protein-II for employing in vaccine development investigations

  Vahideh Valizadeh - Malaria and Vector Research Group (MVRG), Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran,Pasteur Avenue, P.O.BOX 1316943551, Tehran, Iran,
  Sedigheh Zakeri - Malaria and Vector Research Group (MVRG), Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran,Pasteur Avenue, P.O.BOX 1316943551, Tehran, Iran,
  Akram Abouei Mehrizi - Malaria and Vector Research Group (MVRG), Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran,Pasteur Avenue, P.O.BOX 1316943551, Tehran, Iran,
  Navid Dinparast Djadid - Malaria and Vector Research Group (MVRG), Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran,Pasteur Avenue, P.O.BOX 1316943551, Tehran, Iran,

Abstract:

The Plasmodium vivax duffy binding protein-II (PvDBP_II) is a promising blood-stage malaria vaccine candidate.However, the polymorphic nature of this protein is a challenging issue in developing an effective vaccine. In the present study, sequencing analysis was carried out on 63 P.vivax isolates collected from patients in the southeastern regions of Iranduring 2008-2012. Sixteen distinct PvDBP-II haplotypes were detected as a result of arising 20 non synonymous mutations.With regard to high positive value for the ratio of non-synonymous to synonymous mutation rates and also positive valuefor Tajima’s D it was postulated that positive natural selection acts on pvdbp-II gene, however due to excess of rare mutations Fu and Li’s D* and F* tests results in negative values. High selective pressure particularly acted on regions identified as B-cell epitopes or putative IURs. Especially in one region which a high inhibitory B-cell epitope overlaps withan IUR, four prevalent mutations including D384G/E385K/ K386N/Q/ R390H were detected. Additionally based oncalculation of recombination parameters and the decline of R2 index with increasing nucleotide distance, intragenicrecombination may contribute in observed diversity among Iranian P. vivax isolates. Finally to explore whether thesequence diversity of DBP-II causes ineffectiveness of DBP-II based designed vaccine, five different allelic forms of this protein were cloned and expressed in prokaryotic host for further analysis. In conclusion, our results added fundamental information about the nature of P. vivax population circulating in Iran to design an effective PvDBP-II based vaccine.

Keywords:

Vaccine, polymorphism, Plasmodium vivax, Duffy Binding Protein

Perma Link

https://www.civilica.com/Paper-CIGS13-CIGS13_0872.html
COI code: CIGS13_0872

how to cite to this paper:

If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Valizadeh, Vahideh; Sedigheh Zakeri; Akram Abouei Mehrizi & Navid Dinparast Djadid, 2014, Genetic diversity of a vaccine candidate gene encoding Plasmodium vivax Duffy Binding Protein-II for employing in vaccine development investigations, 12th Congress of Iranian Genetics Society, تهران, انجمن ژنتيك ايران, https://www.civilica.com/Paper-CIGS13-CIGS13_0872.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Valizadeh, Vahideh; Sedigheh Zakeri; Akram Abouei Mehrizi & Navid Dinparast Djadid, 2014)
Second and more: (Valizadeh; Zakeri; Abouei Mehrizi & Dinparast Djadid, 2014)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)

Scientometrics

The University/Research Center Information:
Type: Research Center
Paper No.: 679
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.

Research Info Management

Export Citation info of this paper to research management softwares

New Related Papers

Iran Scientific Advertisment Netword

Share this paper

WHAT IS COI?

COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.