Investigation of NOD1 and NOD2 gene expression in coronary artery disease patients in Yazd province

Objective: Coronary artery disease (CAD) is the most common type of heart disease associated with the activation of innate immune TLRs and NOD- like receptor pathways. The NOD1 gene, expressed in cardiac and vascular smooth muscles, is a cytosolic receptor that plays an important role in inflammatory responses by activating the NF-kB pathway. It is involved in many human diseases, but its role and its relationship with the cardiovascular system are less understood. The NOD2 gene is expressed in endothelial cells of the vascular wall and play important roles in promoting vascular inflammation and linking cardiac injury to inflammation. The aim of this study was to investigate NOD1 and NOD2 expression in 13 patients with CAD in Yazd province. Materials and methods: In this Study, we recruited 13 patient and 10 normal control blood samples from Afshar Hospital in Yazd. RNA samples were isolated using Trizol reagent. The mRNA levels of NOD1 and NOD2 genes were determined by Real time PCR. Statistical analysis was performed using t-test.Results: It was revealed that the expression of NOD2 gene in the patients was significantly increased compared to the control group (p<0.05). Moreover, our results demonstrated that although NOD1 expression in patients was more than control group, this difference was not statistically significant (p≥0.05). Conclusion: It can be concluded that NOD2 gene plays an important role in CAD disease. Pharmacological targeting of NOD2-mediated signaling pathways may provide a novel approach to treatment of cardiovascular diseases.

Keywords:

Coronary artery disease , NOD1 , NOD2 , inflammation