Evaluation of BANCR level in CRC tumors and its association with clinical features

Introduction: Colorectal cancer (CRC) is the third known cancer worldwide that has been mostly diagnosed at advanced stages. Therefore, finding new biomarkers with marked sensitivity and specificity is matter of urgent. Studies show that alteration of BANCR expression patternis effective in the initiation and progression of tumorigenesis. So, we evaluated the level of this lncRNA within the CRC patients.Materials and Methods: 52 tumor tissue samples and 10 normal controlswere collected along with from the cases that had been diagnosed and approved by the Taleghani Hospital, Tehran, Iran.Samples were subjected toRNA isolation and then reverse transcripted. The obtained cDNAswere amplified by Realtime-PCR method. Relative expression abundances of BANCR were measured by normalizing to Ribosomal 18S RNA using the 2-∆∆CT method.Results: The data showed a markedupregulation in the expression pattern of BANCR within the tumor samples compared to the controls. These observationswere statistically associated with patient TNM stage, tumor location and differentiation.Conclusion:Taking together, increasing of the BANCRprovokes tumorigenesis in CRC patients. So, this lncRNA could be applied as diagnosis biomarker for cancer detection.

Keywords:

BANCR , Colorectal cancer , Diagnosis biomarker , Tumorigenesis