COI code: CIGS15_343
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors In vitro bone formation of Human Dental Pulp Stem Cells on Zein/Hydroxyapatite scaffoldsMona Zaersabet, - Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
Zivar Salehi, - Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
Mahvash Hadavi, - Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
Soheila Talesh Sasani - Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
Abstract:Functional reconstruction of large osteochondral defects is always a major challenge in articular surgery. Engineering functional bone using combinations of cells, scaffolds and bioactive factors are seen as a promising approach that covers all the attempts to speed up healing of bone in all musculoskeletal disorders. Zein, a native protein derived from corn, has an excellent biodegradability, and therefore becomes a hotspot on research and application in the field of biomaterials. In this study, a zein scaffold was made using the solvent casting/particulate leaching method with sodium chloride (NaCl) particles as the porogen. The microstructural analysis and porosity of the scaffolds were measured by scanning electron microscopy (SEM) and liquid substitution method, respectively. Dental pulp stem cells (DPSCs) were cultured on zein and zein/hydroxyapatite biomaterials. Cell viability and proliferation in scaffolds were analyzed by MTT assay. Using scanning electron microscopy, it was established that both scaffolds had good pore interconnectivity and mean pore size was in the range of 200-300 µm. In addition, the result showed that the zein scaffold had smooth wall surface but HA addition to composite increased surface roughness. This results informed the first step analysis of zein/HA osteoinductive material. Further investigation for scaffold mechanical properties and DPSCs osteogenic differentiation will be evaluated in the future.
Keywords:Zein/hydroxyapatite scaffolds; DPSCs
COI code: CIGS15_343
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Zaersabet,, Mona; Zivar Salehi,; Mahvash Hadavi, & Soheila Talesh Sasani, 2018, In vitro bone formation of Human Dental Pulp Stem Cells on Zein/Hydroxyapatite scaffolds, The Third International and 15th National Genetics Congress, تهران, انجمن علمي ژنتيك ايران, https://www.civilica.com/Paper-CIGS15-CIGS15_343.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Zaersabet,, Mona; Zivar Salehi,; Mahvash Hadavi, & Soheila Talesh Sasani, 2018)
Second and more: (Zaersabet,; Salehi,; Hadavi, & Talesh Sasani, 2018)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
The University/Research Center Information:
Type: state university
Paper No.: 11468
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Attenuation of inflammation genes expression by Juglans regia extract in an Osteoarthritis in vitro like-model.
- Identification of novel short tandem repeats based markers in the expressed sequence of Malus domestica Borkh.
- In silico study of anti-CCHFV effect of lactoferrin from different origin
- Optimization of DNA extraction from different tissues of a medicinal plant, Senecio vulgaris L.
- Design and construction of a new operator: A new attempt to improve protein expression under industrial condition
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.