Enhancement efficiency of callus induction in Tomato Plant tissue culture (Cherry and Olive varieties)
Publish place: The Third International and 15th National Genetics Congress
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
CIGS15_405
تاریخ نمایه سازی: 13 بهمن 1398
Abstract:
For years, plants used for the production of recombinant proteins and as Molecular farming is known. However, the transformation of tomatoes is still not A common method and repeatable for all digits. In this study, we’ve optimized the tissue culture of tomato genotype (Chery) with two cotyledon and hypocotyl explants in 6 different culture medium whit different hormones. In the meantime, culture medium supplemented whit benzyl amino purine (BAP) 3mg/L and Zeatin 1mg/L In addition whit 0.5 mg/L indole acetic acid (IAA) showed the highest regeneration rate. Interaction among Culture medium and explants on callus induction and regeneration were observed. Among the explants cotyledon was the best regeneration.
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Authors
Masomeh Nasiri Mogadam
Department of Agronomy, Faculty of Agriculture, Univresity of Kurdistan, Sanandaj, Iran.
Asad Maroufi
Department of Agronomy, Faculty of Agriculture, Univresity of Kurdistan, Sanandaj, Iran.