JAZF1 Gene Polymorphism Association with the Risk of T2DM in Prostate Cancer Patients

Introduction: Prostate cancer (PCa) and type 2 diabetes mellitus (T2DM) are two of the most common chronic diseases. Epidemiologic studies have shown that there is an association between these conditions. Several mechanisms for this relationship have been proposed, including genetic factors related to T2DM and PCa. Among these factors, juxtaposed with another zinc finger protein 1 (JAZF1) gene is associated with both of them . JAZF1 is a zinc finger protein and inhibits testicular nuclear receptor 4 (TR4) activation through protein-protein interaction, which results in weight loss and alleviates T2DM. In this study, we aimed the association analysis of JAZF1 rs864745 with the risk of T2DM in a group of Iranian PCa patients.Materials & Methods: In this case-control study, 136 subjects were recruited into six groups in order to find any relationship between rs864745 JAZF1 variant and T2DM; Patients only with T2DM (n=23), Normal healthy subjects without history of T2DM and PCa (n=22), Benign prostate hyperplasia (BPH) subjects without history of T2DM (n=23), BPH subjects with T2DM (n=22), Radical prostatectomy (RP) subjects without history of T2DM (n=23) and RP subjects with T2DM (n=23). Genotyping was performed by PCR-RFLP method and confirmed by Sanger sequencing technique.Results: The frequency of the risk alleles for JAZF1 rs864745 between six groups showed no significant association with the risk of T2DM. All analysis was performed by chi2 test and the P-value<0.05 were considered significant.Conclusion: Our study showed that the homozygous state of GG genotype of rs864745 in JAZF1 gene is not associated with T2DM. In conclusion, this genetic variant has no a critical role in reducing the risk of T2DM between patients with PCa. Further investigations should be done in larger sample size and ethnicities.