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Evalition of mir-10a in CLL Patients and Healthy People using RT-qPCR

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Year: 2016
COI code: IPMCMED01_024
Paper Language: English

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Authors Evalition of mir-10a in CLL Patients and Healthy People using RT-qPCR

Monir Sayahian - Department of basic science, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
  Mansoor Salehi - Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Parisa Mohammadinejad - Biology Dept., Shahr e Kord Branch, Islamic Azad University, Shahr e Kord, I.R. of Iran


Background: Chronic Lymphocytic Leukemia (CLL) is the most common human leukemia occurring as indolent or aggressive form. MicroRNAs are small non-coding RNAs involved in several cellular processes and expressed in a tissue-specific manner. In CLL, microRNAs can function as oncogenes or tumor suppressors. The aim of the present study was to compare the expression level of mir-10a in normal and neoplastic samples from CLL patients by RT-qPCR.Methods: The number of 15 blood samples from patients with CLL and 15 blood samples from healthy group under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings were collected. After extracting RNA from normal and tumor blood samples, cDNA synthesis method according to the protocol and RT-qPCR was performed. mir-10a expression level was calculated using Livak method. All data were analyzed using SPSS software.Results: The results of RT-qPCR indicated that miR-10a down-regulation was significantly correlated with CLL cancer clinic pathological features. Expression level of mir-10a was 7.43% in CLL patients and 92.57% in healthy group (p value=0.001).Conclusion: Due to the previous reports, mir-10a act as tumor suppressor in CLL, which could be used as biomarker for CLL diagnosis. In this study we propose that miR-10a is a potential diagnostic marker and therapeutic target of CLL.


Chronic lymphocytic leukemia, microRN

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COI code: IPMCMED01_024

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Sayahian, Monir; Mansoor Salehi & Parisa Mohammadinejad, 2016, Evalition of mir-10a in CLL Patients and Healthy People using RT-qPCR, First Personal Medical Congress, تهران, دانشگاه علوم پزشكي ايران - پژوهشكده ملي مهندسي ژنتيك و زيست فناوري ايران، مركز همكاري هاي فناوري و نوآوري هاي رياست جمهوري, the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Sayahian, Monir; Mansoor Salehi & Parisa Mohammadinejad, 2016)
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