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Evalition of mir-10a in CLL Patients and Healthy People using RT-qPCR

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Year: 2016
COI code: IPMCMED01_024
Paper Language: English

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Authors Evalition of mir-10a in CLL Patients and Healthy People using RT-qPCR

Monir Sayahian - Department of basic science, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
  Mansoor Salehi - Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Parisa Mohammadinejad - Biology Dept., Shahr e Kord Branch, Islamic Azad University, Shahr e Kord, I.R. of Iran

Abstract:

Background: Chronic Lymphocytic Leukemia (CLL) is the most common human leukemia occurring as indolent or aggressive form. MicroRNAs are small non-coding RNAs involved in several cellular processes and expressed in a tissue-specific manner. In CLL, microRNAs can function as oncogenes or tumor suppressors. The aim of the present study was to compare the expression level of mir-10a in normal and neoplastic samples from CLL patients by RT-qPCR.Methods: The number of 15 blood samples from patients with CLL and 15 blood samples from healthy group under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings were collected. After extracting RNA from normal and tumor blood samples, cDNA synthesis method according to the protocol and RT-qPCR was performed. mir-10a expression level was calculated using Livak method. All data were analyzed using SPSS software.Results: The results of RT-qPCR indicated that miR-10a down-regulation was significantly correlated with CLL cancer clinic pathological features. Expression level of mir-10a was 7.43% in CLL patients and 92.57% in healthy group (p value=0.001).Conclusion: Due to the previous reports, mir-10a act as tumor suppressor in CLL, which could be used as biomarker for CLL diagnosis. In this study we propose that miR-10a is a potential diagnostic marker and therapeutic target of CLL.

Keywords:

Chronic lymphocytic leukemia, microRN

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COI code: IPMCMED01_024

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Sayahian, Monir; Mansoor Salehi & Parisa Mohammadinejad, 2016, Evalition of mir-10a in CLL Patients and Healthy People using RT-qPCR, First Personal Medical Congress, تهران, دانشگاه علوم پزشكي ايران - پژوهشكده ملي مهندسي ژنتيك و زيست فناوري ايران، مركز همكاري هاي فناوري و نوآوري هاي رياست جمهوري, https://www.civilica.com/Paper-IPMCMED01-IPMCMED01_024.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Sayahian, Monir; Mansoor Salehi & Parisa Mohammadinejad, 2016)
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