Survey of interaction between macronutrients with selected CETP gene polymorphism in relation with metabolic syndrome

Background: Metabolic syndrom is (MetS) is a complex disorder that is linked to cardiovascular disease and type 2 diabetes. The interaction between genetic and environmental factors, especially diet is the main causes of MetS And we may be able to annul unfavorable effect of genetic changes by altering dietary intake.Aims: to examine the interaction between CETP polymorphisms and macronutrient intake in relation to MetS and its componenets.Methods: In this matched nested case-control study, MetS subjects and controls were selected from of the Tehran Lipid and Glucose Study. Dietary intakes were assessed using semi-quantitative food frequency questionnaire. Anthropometric measurements and biochemical assays were conducted. Single Nucleotide Polymorphism CETP rs3764261, rs5882 were genotyped by the conventional polymerase chain reaction and restriction fragment length polymorphism.Results: A significant interaction between calory density and rs3764261, rs5882 polymorphisms associated with the risk of MetS, high blood pressure and high triglyceride was observed (PiThe risk of metabolic syndrome in carriers of the A (rs3764261) allele decreases by increasing omega-3 fatty acid intake (P trend=0.04). Total fat intake has a significant interaction with rs5882 polymorphism associated with low HDL-C (Pi= 0.02). The ORs of high triglyceride increase significantly in fourth quartiles of total fat and omega3 fatty acid in the CC homozygote, in fourth quartiles of MUFA and trance fatty acid in the A allele carriers of rs63764261 and in fourth quartiles of trance fatty acid in the G allele carriers of and rs5882, compared to first quartiles (P trend<0.05).Conclusion: In the present study the interaction between CETP: rs3764261, rs5882 polymorphisms and the intake of macronutrients in relation to MetS was not significant. There was a significant associations between the rs3764261 CETP SNP and concentration of HDL-C, although there was not a significant interrelation between rs3764261 polymorphism and intake of macronutrients in relation to HDL-C concentration. An interaction between fats intake and rs5882 genotype associated with HDL-C levels was observed.

Keywords:

polymorphism , CETP , gene , diet