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Comparison of two molecular methods for detection of EBF1 gene copies number abnormalities in Patients with Acute Lymphoblastic Leukemia

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Year: 2016
COI code: IPMCMED01_159
Paper Language: English

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Authors Comparison of two molecular methods for detection of EBF1 gene copies number abnormalities in Patients with Acute Lymphoblastic Leukemia

Sahar Mehranfar - Tabriz medical university
Narges Sozangar - School of advanced medical science,tabriz medical university
Farhad Jeddi - School of advanced medical science,tabriz medical university
Abbasali Pou feizi - Hematology & Oncology Research Center, Tabriz Medical Sciences University, Tabriz, Iran

Abstract:

Background: Despite advances in diagnosis, still need a screening method in patients with acute lymphoblastic leukemia (ALL), yet. Our aim is to evaluate Multiplex Ligation-dependent Probe Amplification (MLPA) method ability for screening patients with ALL.Material and Methods: Samples were obtained from 45 childhood patients with B-cell ALL. EBF1 gene copy number abnormalities (CANs) were studied by this method and qPCR. In last we confirm our results by sequencing. Results: From 45 patients with B-ALL, 7 (15%) patients, showed CNAs by MLPA method. 14 (18%) from all mutations, were seen in EBF1 gene and from all these, 3 samples showed changes in exon 10 that were evaluated by qPCR. We used Sanger sequencing as a gold standard method to compare the two methods. Conclusion: Screening mutations of genes involved in acute leukemia remains a challenge in the diagnostic laboratory. MLPA a rapid and valid method for screening genes mutations.

Keywords:

acute lymphoblastic leukemia, copy number abnormality, MLPA, qPCR

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COI code: IPMCMED01_159

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Mehranfar, Sahar; Narges Sozangar; Farhad Jeddi & Abbasali Pou feizi, 2016, Comparison of two molecular methods for detection of EBF1 gene copies number abnormalities in Patients with Acute Lymphoblastic Leukemia, First Personal Medical Congress, تهران, دانشگاه علوم پزشكي ايران - پژوهشكده ملي مهندسي ژنتيك و زيست فناوري ايران، مركز همكاري هاي فناوري و نوآوري هاي رياست جمهوري, https://www.civilica.com/Paper-IPMCMED01-IPMCMED01_159.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
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