ANTIBACTERIAL ACTIVITY OF JUNIPERUS POLYCARPUS AND GERANIUM ROBERTIANUM LEAF EXTRACTS AGAINST PATHOGENIC STAPHYLOCCOCAL STRAINS
Publish place: 19th International Congress of Microbiology of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
MEDISM19_186
تاریخ نمایه سازی: 13 مهر 1397
Abstract:
Background and Aim:Staphylococcus epidermidis, the main pathogen in implant-related infections, is common bacteria of human skin that form biofilms on the surface of surgical implants. Staphylococcus aureus is a gram positive, commensal bacterium that colonize the human skin, nasopharynx and gastrointestinal tract which causes a variety of localized and invasive infections such as bacteremia, osteomyelitis, arthritis and prosthetic joint infections. Juniperus genus has 6 species in Iran and is used in traditional medicine for wound healing, diabetes, tuberculosis, pneumonia and gastric ulcer. Geranium genus is used as an antidiarrheal, anti-inflammatory and antidiabetic. The aim of this study was to investigate the possible antibacterial properties of leaf extract of these plants.Methods:methanolic extracts of freeze-dried plant leafs were prepared by Perculation method. Then Serial dilution of herbal extracts from 0.19 to 100mg/ml in distilled water and 5% DMSO were prepared and the Minimum Inhibition Concentrations (MICs) were performed according to the CLSI standard protocols in 96-well microplates. The plates were titrated with TTC (Triphenyl tetrazolium chloride)-dye after 24hours of incubation to determine the MIC value.Results:the outcomes revealed that the MICs of both extracts had the same results for S. aureus and S. epidemidis that was 1.57mg/ml and 12.5mg/ml respectively. The Minimum Bactericidal Concentration (MBC) of Geranium extract for S. aureus and S. epidemidis was obtained 12.5mg/ml and 3.12mg/ml respectively, and for the Juniperus extract was obtained 25mg/ml for both bacteria.Conclusion:according to this study, both leaf extracts demonstrated high bacteriostatic and bactericide properties which could be great candidates for staphylococcal infections treatment.
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Authors
Abolfazl Shirmohamadi
Department of Biology, Faculty of Science, Ferdowsi university of Mashhad, Mashhad, Iran
Abolfazl Shirmohamadi sosfad
Department of Biology, Faculty of Science, Ferdowsi university of Mashhad, Mashhad, Iran
Mohammad Reza Sharifmoghadam
Department of Biology, Faculty of Science, Ferdowsi university of Mashhad, Mashhad, Iran
Masumeh Bahreini
Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran