ISOLATION MOLCULAR AND IDENIFICATION OF MYCOBACTERIUM PORCINUM AND MYCOBACTERIUM CELERIFLAVUM FROM MARKAZI PROVINCE ENVIRONMENTAL RESOURCES AND ANALYSIS OF THEIR POLYCYCLIC AROMATIC HYDROCARBONS’ BIODEGRADATION ACTIVITY

Background and Aim:Polycyclic aromatic hydrocarbons are one of the prevalent oil pollutant. Nowadays these carbohydrates due to their Toxicity, mutagenesis, carcinogenicity, and also environmental stability caused by hydrophobia character and low solubility are considered as one of the preferences of environmental protection agancy. Therefore, clearance of the regions polluted with these compounds are of significance. Biodegradation of these compounds is a safe and affordable method of environmental clearance. In this research, we described the molcular isolation and identification of the mycobacteruim’s strains abling to degrade polycyclic aromatic hydrocarbons in vitro.Methods:a total of 30 environmental samples colected from the contaminated sites of Markazi province have been analysed by using the combined microbiologic and molcular methods such as PCR and Genetic makers sequencing of 16SrRNA, hsp65 and rpoB. The growth rate in presence of pollutants, chromatography and turbidity have been applied In order to determine the biodegradation activities of the isolates.Results:6 isolates of mycobacteruim ( 20% ) have been isolated from 30 samples which 4 and 2 isolates appertained to 2 species of mycobacteruim porsinum and mycobacteruim selifravom respectively. The strains of mycobacteruim porsinum and seflivarom could degrade 50% and 70% of PAH in concentration of 1 mg PAH, successfully.Conclusion:The results achived in this research illustrated that the isolated strains of mycobacteruim porsinum and mycobacteruim selriflavom have a high ability to biodegrade the polycyclic aromatic hydrocarbins. Hence, additional investigations are recomended for isolation and applicationally use of the bacteria’s strains for biological deletion of polycyclis aromatic hydrocarbons from contaminated environments.

Keywords:

Mycobacterium , Bioremediation , 16S rRNA sequencing , Phylogeny