Cytotoxic study of silver nanoparticles synthesized from rubia tinctorum on ht29 cell line

Cancer is a collection of diseases involving anomalous cell growth with the dormant to invade or spread to other parts of the body. Silver nanoparticles (AgNPs) are one of the most basic and attractivenanomaterials among numerous metallic nanoparticles that are involved in biomedical applications. AgNPs play an important role in nanotechnology, particularly in nanomedicine. Although several noblemetals have been used for various purposes, AgNPs have been focused on potential applications in cancer scrutiny and healing.In the present study, we investigated the potential cytotoxic effect of silvernanoparticles (Ag NPs) on human colon adenocarcinoma cell line HT29 cells. The fatal cells were seeded in 96 well plate after to achieve the logarithmic phase. After 24h of incubation, the cells wereexposed to various concentrations (12.5, 25, 50 and 100 μg/ml) of Ag NPs nanoparticles. In order to evaluate the effect of time on the toxicity induced by the nanoparticle, 24, 48 and 72 h after treatment,the toxicity was evaluated using MTT. After incubation, the treatment medium was evacuated and the MTT solution was added to each of the wells for 4 h. Then, in order to dissolve the formazan crystals,100 μl DMSO was added to each well. Finally, the optical absorption at 570 nm was evaluated by (Epoch, USA) spectrophotometer. The event of Ag NPs nanoparticles on HT29 exhibits a dose- dependent toxicity for the cell tested and the viability of HT29 cells decreased to 50 % (IC50) at the concentration of 50 μg/ml. Ag NPs nanoparticles synthesized from Rubia tinctorum extract can be used as potential anticancerous agent

Keywords:

Colon Cancer , Cell and Cancer , Cancer Genetics , Targeted Cancer Therapy , Drugs and Cancer