Cytotoxic effects of zinc oxide nanoparticles biosynthesized with ceratonia siliqua l extract on mcf-7 breast cancer cell line

One of the deadly cancers that threatens the lives of women is breast cancer that is the second leading cause of death from cancer in world. Apoptosis is an important phenomenon in chemotherapy and the elimination of cancer cells. Nanoparticles with cytotoxic effects and apoptosis inducers are considered as potential therapists. A new approach to the synthesis of nanoparticles is the use of plant extracts andthe synthesis of nanoparticles in green. Green-synthesized nanoparticles have good biological and therapeutic properties . Some studies show that the zinc oxide nanoparticles (ZnO-NPs) haveantibacterial, anti-angiogenesis and anti- cancer activity. The purpose of this study was to examine the cytotoxic effects of ZnO-NPs biosynthesized with Ceratonia siliqua L extract (ZN-NPs/CS) on MCF-7 breast cancer cells.Mcf7 cells were cultured in 96 plates with density about 5000 cells to each wells ( RPMI medium containing 10% FBS). After 24 hours that the cells found normal morphology and attached to the bottom of the plate, they were treated with the concentrations of the ZN-NPs/CS and incubated for of 24, 48 and 72 hours. The MTT solution was added to each wells and after 4 hours ofincubation and adding the DMSO on the cells, absorbance was measured.The obtained analysis displayed the declining percentage of MCF-7 survival rate together with increasing doses of ZN-NPs/CS. The viability of malignant cells in the presence of ZnO-NPs has significantly decreased with IC50 about 50, 40 and 15.6 μg/ml doses dependent manner in 24, 48, and 72 hr comparative to the control groups. Thus, the results obviously suggested that ZN-NPs/CS capable of inhibiting cancer cells in dose and time-dependently. Zinc Oxide nanoparticles biosynthesized with Ceratonia siliqua L extract have the ability to induce apoptosis in MCF-7 cells in time and dose-depended manner and they can be considered as a promising strategy for the treatment of breast cancer.

Keywords:

Breast Cancer , Gene and Cancer , Cell and Cancer , Lifestyle and Cancer , Drugs and Cancer