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title

Effect of silver nanoparticles on acrosome reaction in rat

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Year: 2012
COI code: NCNCMB01_075
Paper Language: English

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Authors Effect of silver nanoparticles on acrosome reaction in rat

Fersshteh Sadat Younesi - Yazd-ACER higher education institute; engineering faculty, Yazd, Iran
Mohsen MiresmaeiliSayyed - Yazd-ACER higher education institute; engineering faculty, Yazd, Iran
Narges Nikounahad - Yazd-ACER higher education institute; engineering faculty, Yazd, Iran

Abstract:

Background:gametogenesis is a biological process that is sensitive to environmental agents. Nanoparticles have nano scale diameter and they can across through cell membrane. Despite the wide application of them, there is lack information about their toxicity on spermatozoa Objective: the aim of present study is evaluate the silver nanoparticles on acrosome of spermatozoa Materials and Methods:In this case-control study, in experimental group, 24 malewistar rats (purchased from Yazd medical faculty)(8 rats/group) received oral feeding silver nanoparticles every 12 hours in one spermatogenesis period (48 days) by gavage in 25, 50 and 100 mg/kg concentration. Control group (8 rats) were treated on schedule with distilled water.Then rat's spermare stained by triple staining protocol for acrosome reaction Results: all dose of silver nanoparticles shown negative effect on sperm's acrosome. Our result shown significant difference (P<0.05) between control group and experimental groups. Also, dose-dependent increases in silver nanoparticle in experimental groups were observed Conclusion: the results suggested that silver nanoparticle may across through sperm's membrane and induce acrosome reaction. Also these particles stimulate cell apoptosis

Keywords:

silver nanoparticle, sperm, acrosome reaction

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COI code: NCNCMB01_075

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Younesi, Fersshteh Sadat; Mohsen MiresmaeiliSayyed & Narges Nikounahad, 2012, Effect of silver nanoparticles on acrosome reaction in rat, 1st National Conference on New Cellular Molecular Biotechnology, پرند, دانشگاه آزاد اسلامي واحد پرند, https://www.civilica.com/Paper-NCNCMB01-NCNCMB01_075.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Younesi, Fersshteh Sadat; Mohsen MiresmaeiliSayyed & Narges Nikounahad, 2012)
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