Donor Affect Tenogenic Differentiation of Equine Adipose Mesenchymal Stem Cells ASCs

Background: The advent of regenerative medicine has brought us the opportunity to regenerate, modify and restore organs function. Stem cells, a key resource in regenerative medicine, can generate into one or more specialized cell types like teno-cytes. Before clinical application of stem cells, their character-istics should be determined. This study designed to investigate weather donor has effect on tenogenic differentiation of equine adipose mesenchymal stem cells (ASCs) in response to platelet-derived growth-factor (PDGF).Materials and Methods: Equine ASCs derived from three horses were used in this experiment. ASCs at passage 5 were cultured with basic culture medium containing PDGF-BB (20 ng/ml), L-Prolin and ascorbic acid (AA) at 37°C under 5% CO2 for 14 days. The cells in control group, were cultured with only basic culture medium. At the end, Sirius Red and H&E stain-ing were used to assess collagen production, as a extracellular matrix protein for tendon, and cell morphology.Results: ASCs in treatment groups were changed to elongated spindle shape like tenocytes. The results of Sirus Red staining revealed that the production of collagen has been elevated in treatment groups compared with control groups significantly. Statistical analysis showed that the production of collagen is different among ASCs derived from 3 horses.Conclusion: Our data revealed that cell differentiation toward tenocytes was different for each horses. In other words, donor affect tenogenic differentiation potential of equine Ascs.

Keywords:

Equine , Mesenchymal Stem Cells , Donor , Tenogenic Differentiation