Evaluation of Mummy on the Function of HUVEC Cells and Fibroblast in skin wound Healing

In recent decades, due to the advancement of modern methods in wound healing and the desire to use the herbal remedy as complementary medicine has made it possible for physicians to discuss and seriously challenge in the field of patients skin wounds and healing them. Therefore, the purpose of this study is to investigate the effect of mummy on HUVEC cell line and fibroblasts. In this study, The mummy was applied on HUVEC cell line and fibroblasts at 31.25, 62.5, 125, 250, 500, 1000, and 2000 mg / ml concentrations and the mortality rates of the cells was examined at three durations of 24, 48, and 72 hours after the incubation using the MTT test and compared with the control group. Results of the MTT test showed that, the viability of HUVEC cells in concentrations of 500 to 2000 mg / ml in 24-hour incubation, decreased significantly P <0.01 and P <0.001 respectively. Also, the percentage of viable cells increased than the control group in concentrations of 31.25 to 1000 mg / ml and 500 mg / ml at 48 h (P <0.01 and P <0.001) and 72 (P <0.001) hours, respectively. In the HF2FF cells level, the survival of the cells in concentrations of 250 to 2000 mg / ml of mummy material at all three incubation time (24, 48 and 72 hours) had a significant decrease compared to control, which indicates the toxicity of the mummy substance in these concentrations. The findings from our result show that mummy in different concentrations of endothelial cells over the periods of 48 and 72 hours not only caused the cell cytotoxicity, but also increased the proliferation of endothelial cells, except in the high concentration. In fibroblast cells, the mummy can have a stimulant effect on the growth and survival of fibroblastic cells at low doses, which will ultimately result in facilitate and accelerate skin regeneration. It has a side effect on the cells at high concentrations due to being toxic

Keywords:

Mummy , Wound Healing , Fibroblast Cells , HUVEC Cells