Investigating the Effects of Morphine on Survival and Sensitivity to Cisplatin in Ovarian Cancer Cells
Publish place: Journal of Cell and Molecular Research، Vol: 11، Issue: 1
Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
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JR_JCMR-11-1_001
تاریخ نمایه سازی: 29 اسفند 1398
Abstract:
Morphine as an analgesic drug is used frequently in cancer patients. Contradictory results have been achieved from previous studies related to morphine effects in different concentrations. In current study, we examined the effect of clinical concentrations of morphine on A2780Cp cell line related to ovarian cancer. Moreover, its effect on the cytotoxicity of cisplatin was investigated. A2780CP cells were cultured in RPMI1640 medium and treated with clinical doses of morphine alone or in combination with cisplatin. The rate of cell proliferation was measured using MTT assay, morphological changes of nuclei were revealed by 4′,6-diamidino-2-phenylindole (DAPI) staining, and expression of B-cell lymphoma 2 (Bcl-2) was measured using flowcytometry. MTT assay results showed clinical concentration of morphine had no effect on viability of A2780CP cells and toxicity of cisplatin. DAPI staining revealed no chromatin condensation in presence of morphine, and flowcytometry analysis showed that the expression of Bcl-2 in treated cells did not differ from control cells. In accordance with findings in other kinds of cancer, our results demonstrated that morphine did not interact with the function of cispatin in ovarian cancer. This finding can be considered in clinical applications of morphine.
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Authors
Maryam Rezaeigazik
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Mohammad Nabiuni
Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Hanieh Jalali
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Majid Kabuli
Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran