The alterations of thyroid hormones in Epinephelus coioides affected by Vibrio alginolyticus

The aim of this study was to investigate the infectious stressor (V. alginolyticus) onthe balance of thyroid hormones (Triiodothyronine (T3) and thyroxine (T4)) inOrange-spotted grouper (Epinephelus coioides). To perform this study, 50specimens of immature groupers with mean length of 20.8 ± 0.2 cm were selectedand randomly divided into two groups. First group received no injection as controland second one injected intraperitoneally with a concentration of 107 cfu/ml ofbacteria. Fish blood samples in days 1, 4, 7 and 14 were taken.40T The samples werecentrifuged about 10 minutes in 3000*g to separate the serum. The s.erum level ofthyroid. hormones. .were measured .using ELISA. The results showed a significantdecrease in the levels of thyroid hormones in the day 7 compared to those of earlierdays (P<0.05). But, the levels of both hormones increased upto the end of experiment,although, it still was lower than the control group (P<0.05).

Keywords:

Vibrio alginolyticus , Epinephelus coioides , Thyroid , riiodothyronine , Thyroxine